![]() The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.Īngiotensin II is thought to participate in aneurysm formation, because of its ability to induce and perpetuate inflammation in the aortic wall. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined. The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. ![]() In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Īn optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Ī molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. However, the clinical use of ILE for this purpose needs more evaluation to determine its exact indication and safety. In conclusion, ILE could reverse HAL-induced hypotension same as the other lipophilic drugs. ![]() ILE 20% at the dose of 18 ml/kg could return the reduced mean arterial pressure and diastolic blood pressure sooner than the other doses and normal saline. We measured blood pressure at 0, 0.5, 1, 2, 3, 4, 8, and 24 h after starting HAL administration, from left forelimb using a noninvasive method that was carried out automatically with a neonatal intensive care unit bedside monitor. The other three groups received ILE 20% solution (6, 12, and 18.6 ml/kg) following HAL (2.6 mg/kg) administration. The third group received 18.6 ml/kg normal saline after HAL infusion (2.6 mg/kg). Two groups received aseptic distilled water intravenously followed by infusion of 18.6 ml/kg normal saline, as negative control group, or ILE 20% after 0.5 h. The aim of this study was to investigate the beneficial hemodynamic effects of ILE on acute HAL poisoning. Hypotension is an important adverse effect of HAL administration and overdose. Haloperidol (HAL) is a butyrophenone antipsychotic agent which is highly lipophilic. There are many reports on the effects of intravenous lipid emulsion (ILE) as an antidote in drug toxicity. ![]()
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